Dynamics of hematological parameters, macromorphological and radiological results of reparative osteogenesis in rabbits for the use of platelet concentrates and hydroxyapatite ceramics

The results of the dynamics of the morphological parameters of the blood of a rabbit with model defects of the opening of the spongy and compact bone tissue at di erent periods of reparative osteogenesis are presented. Formed 4 groups of rabbits. Bone lesions in the rst experimental group were lled with injectable platelet-rich brin, in the second - platelet-rich brin, in the third - a combination of platelet-rich brin, and hydroxyapatite with β-tricalcium phosphate.In the control group, the defects remained healed under a blood clot. All animals were in the same conditions of feeding and keeping, had unlimited access to water. During the study, rabbits were clinically observed. Blood was taken for morphological examination before surgery and on the 3rd, 7th, 14th, 21st, 42nd day. Animals were taken from the experiment on the 14th, 21st and 42nd days, an X-ray examination was carried out, bone tissue samples were taken. It was established that trauma of bone tissue leads to a number of reactions of the body aimed at restoring the damaged area. Against the background of the general picture of the obtained morphological results, the level of platelets signi cantly changes compared to the physiological norm, while the number of red blood cells and white blood cells does not go beyond it. There is a slight increase in hemoglobin levels, especially in the experimental groups on the twenty- rst and forty-second day. Signi cant changes in the number of red blood cells, white blood cells, platelets and hemoglobin concentration in the experimental groups were noted compared with the control group at di erent periods of reparative osteogenesis.The use of various types of platelet concentrates a ects the overall reaction of the body. On radiographs of the radial bones (compact bone tissue) on the twenty- rst day in the second and third experimental groups, where brin enriched with platelets and its combination with hydroxyapatite materials with β-tricalcium phosphate were used to replace bone defects, the in ammatory reaction was manifested to a lesser extent. There was no signi cant formation of bone callus compared with the rst, in which model defects were lled with injectable platelet-rich brin and the control group. Each of the types of platelet concentrates and their combination with hydroxyapatite ceramics have a di erent e ect on the restoration of bone defects and is accompanied by the appearance of a number of reactions, both local and general. According to the degree of intensity of this process, they can be placed in the following sequence: i-PRF ˂ PRF ˂ PRF+GT. Macromorphologically and radiologically signi cant di erences were not detected for di erent types of bone tissue due to the use of each of the substances that concentrates platelets. The combination of hydroxyapatite granules with β-tricalcium phosphate and platelet-rich brin proved to be the best option for repairing the damaged area. Its use provides a signi cantly smaller manifestation of the local in ammatory reaction and causes the formation of optimal bone marrow.Key words: PRF, i-PRF, granules, centrifuges, growth factors, bone marrow.