Open AccessHuman mulipotential mesenchymal/stromal stem cells are derived from a discrete subpopulation of STRO-1bright/CD34 /CD45 /glycophorin-A-bone marrow cells
Author(s) -
Andrew C.W. Zannettino,
Sharon Paton,
Angela Kortesidis,
Fiona Khor,
Silviu Itescu,
Stan Gronthos
Publication year - 2007
Publication title -
haematologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.782
H-Index - 142
eISSN - 1592-8721
pISSN - 0390-6078
DOI - 10.3324/haematol.11691
Subject(s) - cd34 , stromal cell , glycophorin , bone marrow , mesenchymal stem cell , clonogenic assay , stem cell , haematopoiesis , flow cytometry , progenitor cell , pathology , biology , cancer research , immunology , medicine , microbiology and biotechnology , cell , antigen , genetics
Magnetic and flow cytometry-based methods were used to characterize clonogenic stromal cells in human bone marrow. STRO-1(bright) stromal cells were found to lack expression of CD34, CD45 and glycophorin-A markers associated with hematopoietic progenitor cells. These studies support the view that these are two distinct stem cell compartments in adult bone marrow.