Open AccessLoss of heterozygosity in acute leukemia: evidence of frequent submicroscopic deletions
Author(s) -
Cecilia Agueli,
Rosaria Basiricò,
Francesco Fanfani,
Valentina Rizzo,
Lucia Cascio,
Giuseppe Cammarata,
Anna Marfia,
Massimo La Rosa,
Salvo Mirto,
Alessandra Santoro
Publication year - 2007
Publication title -
haematologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.782
H-Index - 142
eISSN - 1592-8721
pISSN - 0390-6078
DOI - 10.3324/haematol.11028
Subject(s) - loss of heterozygosity , biology , myeloid leukemia , microsatellite , genetics , leukemia , acute leukemia , cancer research , gene , allele
Although chromosomal abnormalities are detected by conventional cytogenetic analysis (CCA) in 40-60% of patients with acute myeloid leukemia (AML), cryptic chromosomal deletions may only be detected by molecular analysis. To determine their frequency, we studied 74 cases of AML by microsatellite allelotype assay using 35 microsatellites spanning eight chromosomal regions known to be frequently involved in AML. In 42 (57%) we found DNA imbalance at the screened loci. This was detected by CCA only in 4 cases. Our data show that cryptic deletions are a common event in AML.