Open AccessPendekatan PCR-RAPD dalam Menentukan Kekerabatan dan Konservasi Padi Varietas Lokal Sumatera Selatan
Author(s) -
Fickri Adriansyah,
Laila Hanum,
Muharni Muharni,
Yuanita Windusari
Publication year - 2019
Publication title -
jurnal lahan suboptimal: journal of suboptimal lands
Language(s) - English
Resource type - Journals
ISSN - 2302-3015
DOI - 10.33230/jlso.7.1.2018.347
Subject(s) - rapd , primer (cosmetics) , biology , germplasm , microbiology and biotechnology , botany , genetic diversity , chemistry , medicine , population , environmental health , organic chemistry
Adriansyah et al, 2018. Polymorphism Analysis of Local Varieties of South Sumatra Rice Based on PCR-RAPD. JLSO 7(1):The existence of rice germplasm is threatened because of the introduction of various high-yielding varieties. This study aimedto describe variation in local varieties of South Sumatra rice using RAPD. Rice DNA was isolated using the Promega Wizard Purification Systems KIT. Primers used in the study was OPA-9, OPA-10, OPA-13, and OPA-16. All primers could amplify DNA with optimal quality. Each primer produced different number of DNA bands in which 5, 9, 12 and 9 DNA bands were produced by OPA-9, OPA-10, OPA-13, and OPA-16 primer, respectively.