Open AccessAssessing operational inactivation of horseradish peroxidase during Brilliant Blue G decolorization
Author(s) -
Delia MĂRTINAȘ,
Mihaela Puiu,
Petruţa Oancea,
Adina Răducan
Publication year - 2021
Publication title -
revue roumaine de chimie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.135
H-Index - 21
ISSN - 0035-3930
DOI - 10.33224/rrch.2021.66.3.06
Subject(s) - horseradish peroxidase , hydrogen peroxide , oxidizing agent , chemistry , substrate (aquarium) , peroxidase , degradation (telecommunications) , bicarbonate , catalysis , kinetics , chromatography , nuclear chemistry , photochemistry , enzyme , biochemistry , organic chemistry , biology , ecology , telecommunications , physics , quantum mechanics , computer science
An efficiency study about the horseradish peroxidase (HRP) activity over Brilliant Blue-G (BB) decolorization with hydrogen peroxide was achieved through kinetic analysis. Differential and extended kinetic curves were used for assessing the inactivation paths and for optimizing the overall degradation process. The catalytic and the specificity constants estimated via Michaelis-Menten model demonstrated that HRP is highly efficient in dye decolorization, although operational inactivation due to formation of degradation products still occurred. It was noticed that the bicarbonate anions exerted protective effects on the enzyme inactivation caused by the oxidizing substrate but affected less the inactivation due to the degradation products of BB.