Open AccessRole of proteinuria in the regulation of renal renin-angiotensin system components in unilateral proteinuric rats
Author(s) -
Leo E. Deelman,
Gerjan Navis,
Erik de Boer,
Mirian Wietses,
Dick de Zeeuw,
Robert H. Henning
Publication year - 2003
Publication title -
jraas. journal of the renin-angiotensin-aldosterone system/journal of the renin-angiotensin-aldosterone system
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.457
H-Index - 46
eISSN - 1752-8976
pISSN - 1470-3203
DOI - 10.3317/jraas.2003.006
Subject(s) - endocrinology , medicine , renin–angiotensin system , kidney , messenger rna , proteinuria , renal function , receptor , angiotensin ii , angiotensin converting enzyme , biology , blood pressure , biochemistry , gene
Renin-angiotensin system (RAS) overactivity has been implied in progressive renal function loss. We investigated whether changes in the renal expression of RAS components are specifically associated with the proteinuric kidney. Unilateral adriamycin-induced proteinuria was obtained by clamping the left renal artery before injection of adriamycin. In control animals, both left and right renal arteries were clamped. Twelve weeks later, mRNA expression of RAS components was determined in both kidneys. In the affected and non-affected kidney of the unilateral proteinuric rat, we demonstrate up-regulation of angiotensin-converting enzyme (ACE) mRNA (213%±22 and 188%±24 of controls, respectively), up-regulation of transforming growth factor (TGF- ) mRNA (956%±229 and 418%±56) and down-regulation of angiotensin type 2 receptor (AT 2 -R) mRNA (24%±5 and 20%±5). The expression of angiotensin type 1 receptor (AT 1 -R) mRNA and inositol 1,4,5-trisphosphate receptor type I (IP 3 R-I) mRNA were unchanged. In conclusion, renal expression of ACE, AT 2 -R, and AT 1 -R mRNA is not mediated by protein leakage. Local intrarenal protein leakage did influence renal TGF- mRNA expression.