Open AccessEssential Elements as Biomarkers of Metastatic Renal Cell Carcinoma
Publication year - 2020
Publication title -
medical and clinical research
Language(s) - English
Resource type - Journals
ISSN - 2577-8005
DOI - 10.33140/mcr.05.07.04
Subject(s) - renal cell carcinoma , lung , medicine , carcinoma , plasma concentration , inductively coupled plasma mass spectrometry , pathology , oncology , cancer research , chemistry , mass spectrometry , chromatography
Renal cell carcinoma (RCC) represents 3% of human malignant tumors and approximately 90% of malignant renal neoplasms.Despite great therapeutic advances in the last decade, metastatic RCC (mRCC) is still considered an incurable disease. Inthis study, we examined the potential of essential elements as biomarkers of mRCC using an orthotropic metastatic mousemodel. Frozen lung and plasma samples from healthy and mRCC-induced mice were lyophilized, digested, and analyzed usinginductively coupled plasma mass spectrometry. In metastatic lungs, a significant increase in Ca concentration (268%) wasobserved, whereas a significant decrease in Cu (23.2%), Fe (17.4%), Mn (38.8%), and Na (11.7%) was observed. The plasmaof mRCC-induced mice showed decreased concentrations of Mn (53%), Na (19.7%) and Zn (49,50%) and increased levels ofCa (53%), Cu (39.5%), Our findings revealed marked differences in the concentrations of essential elements in the lung andplasma of the metastatic mouse model. The circulating levels of Ca, Cu, Mn, Na, and Zn could be utilized as diagnostic andtherapeutic response biomarkers.