Open AccessSaponins and Flavonoids from Ludwigia leptocarpa (Nutt) Hara (Onagraceae): Isolation, Characterization, Antibacterial and Antioxidant Activities
Publication year - 2019
Publication title -
medical and clinical research
Language(s) - English
Resource type - Journals
ISSN - 2577-8005
DOI - 10.33140/mcr.04.07.01
Subject(s) - shigella flexneri , antibacterial activity , dpph , vibrio cholerae , minimum bactericidal concentration , minimum inhibitory concentration , shigellosis , antioxidant , traditional medicine , microbiology and biotechnology , chemistry , antimicrobial , shigella , biology , bacteria , escherichia coli , biochemistry , medicine , genetics , gene
Diarrhea continues to be one of the most common causes of morbidity and mortality among infants and children in developingcountries. The most common microorganisms responsible for diarrhoeal diseases are Vibrio cholerae, Escherichia coli andShigella spp. The present study aims to evaluate the antibacterial and antioxidant activities of extracts and compounds fromLudwigia leptocarpa, a plant traditionally used for its vermifugal, anti-dysenteric and antimicrobial properties. The MeOHextract was prepared by maceration from dried whole plant and successively extracted with ethyl acetate and n-butanol toobtain EtOAc and n-BuOH extracts respectively. The column chromatography of the EtOAc and n-BuOH extracts followed bypurification of different fractions led to the isolation of six known compounds. Structures of isolated compounds were assignedon the basis of spectra analysis, and by comparison with those from the literature. The antioxidant activity was evaluatedby the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and trolox equivalent antioxidant capacity (TEAC) assays. The antibacterialactivity was assessed by performing minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC)against the strains of Gram-positive bacterium, Staphylococcus aureus (a major cause of community and hospital-associatedinfection), and Gram-negative multi-drug resistant bacteria, Vibrio cholerae (causative agent of cholera) and Shigellaflexneri (causative agent of shigellosis). All of the extracts showed different degrees of antioxidant and antibacterial activities.Compounds 2, 3 and 6 displayed the largest antibacterial and antioxidant properties which were in some cases equal orhigher than those of reference drugs. The overall results of the present study shows that L. leptocarpa has potentials as asource of natural anti-diarrhoeal and anti-free radical products, given further investigations.