Open AccessInvitro Cytotoxicity and Glucose Uptake Activity of Mushroom Pleurotus Eous in Methanol Extract Using L-6 Cell Line
Publication year - 2019
Publication title -
medical and clinical research
Language(s) - English
Resource type - Journals
ISSN - 2577-8005
DOI - 10.33140/mcr.04.01.03
Subject(s) - cytotoxicity , mtt assay , mushroom , glucose uptake , cell culture , chemistry , biochemistry , in vitro , viability assay , insulin , pharmacology , biology , food science , microbiology and biotechnology , genetics
The aim of the present study is to evaluate the cytotoxicity and glucose uptake activity of methanolic extract of pleurotus eousmushroom using L6 cell lines. An MTT assay is a colorimetric assay based on assessing the cell metabolic activityThe resultsshowed that the extracts did not confer any cytotoxicity. The effects were observed in treated cells up to 6.25 mg/ml extract asmeasured with MTT. Also the mushroom extracts shows better glucose uptake potential. The results were compared with Insulina standard antidiabetic drug. Insulin enhance the glucose uptake upto 97.8%. The L6 cell lines enhance the glucose uptake by57.4% at 65µg concentration.