Open AccessScreening and Partial Characterization of Natural Antioxidants from Seaweeds Collected From, Rameshwaram Southeast Coast of India
Publication year - 2020
Publication title -
journal of marine science research and oceanography
Language(s) - English
Resource type - Journals
ISSN - 2642-9020
DOI - 10.33140/jmsro.03.01.01
Subject(s) - dpph , chemistry , acetone , phenols , antioxidant , botany , chlorophyta , food science , methanol , scavenging , dry weight , algae , traditional medicine , biology , biochemistry , organic chemistry , medicine
The aim of this work is to estimate in-vitro antioxidant activities such as total antioxidant capacity, free radicals (DPPH)scavenging activity, hydrogen peroxides (H2O2) scavenging activity, total reducing power scavenging activity and total phenols,of various extracts of seaweeds such as aqueous, acetone, ethanol, methanol, and pigments content by spectrophotometricmethod, of 33 seaweeds species among which 11 Chlorophyta, 11 Phaeophyceae and 11 Rhodophyta, collected during2016 from two stations viz. Olaikuda and Vadakkadu, at Rameshwaram, southeast coast of India. Among four differentextracts aqueous extracts from all seaweeds had minimum activity than acetone, methanol and ethanol. The Rhodophytaand Phaeophyceae had high antioxidant activity in comparing to Chlorophyta. The highest total antioxidant activity wasfound in acetone extract from Turbinaria decurrens (98.97±0.00%), followed by its methanol extract (98.81±0.60%) andethanol extract (98.58±0.53%). The highest reducing power and H2O2 scavenging activity were found in acetone extractof Caulerpa racemosa (383.25±1.04%), and methanol extract from Caulerpa racemosa var. macrophysa (24.91±0.49%).The methanol extract from Caulerpa scalpelliformis contained the highest total phenol (85.23±0.12%). The Chloro-a andChloro-b contents were the highest in Gracilaria foliifera (13.69±0.38% mg/gm dry wt.) and Caulerpa racemosa var.macrophysa (9.12 ±0.12% mg/gm dry wt.) likewise carotenoid was also the highest in Gracilaria foliifera (0.054±0.0003%mg/gm dry wt.) and Caulerpa racemosa var. macrophysa (0.04 ±0.002% mg/gm dry wt.). The partial characterization ofthe extract which contents the highest activity was done by UV-Visible Spectrophotometer, FTIR, and NMR. The functionalgroups and all the possible compounds present in the extract were partially characterized. It can be concluded from thisstudy, that some seaweed extract can be used for natural antioxidant production, after further characterization to negotiatethe side effect of synthetic, market available antioxidants.