In Vitro Antioxidant Activity of Sesame Milk Fermentation in Human Low Density Lipoprotein (LDL)

Lignan sesaminol triglucoside is a bioactive compound in sesame milk that showed higher antioxidant activity after it ishydrolyzed by β-glucosidase. The objectives of this study were to determine in vitro antioxidant activity of fermented sesamemilk (FSM) extract and sesame milk (SM) extract against DPPH and LDL oxidation and to examine phenolic, sesaminoltriglucoside content and β-glucosidase activity. Antioxidant activity was examined using DPPH and TBARS assay with LDL asthe oxidation substrate. Sesaminol triglucoside was identified with HPLC diode array detector and β-glucosidase activity wasdetermined by measuring hydrolysis rate of ρ-nitrophenyl-β-D-glucopyranoside (ρNPG). After fermentation of sesame milk withL. plantarum Dad 13, the β-glucosidase activity was 70.3±0.023 mU/mL fermented sesame milk, the sesaminol triglucosidecontent of SM and FSM were 5.65 to 2.56 mg/100 mL of sesame milk, respectively, the phenolic content of SM and FSM were3.81±0.10 and 7.9±0.08 mg GAE/g dry sesame seed, respectively, radical scavenging activity of SM and FSM 18±0.64, and45.5±0.37% respectively. Fermented sesame milk inhibited human plasma LDL oxidation by 1.82 compared to unfermentedsesame milk. This result related to hydrolysis of sesaminol triglucoside by β-glucosidase which was produced by L. plantarumDad 13 that resulted in sesaminol. This results suggest that fermented sesame milk extract has in vitro antioxidant activity inhuman LDL better than sesame milk extract