Open AccessStructural and Functional Prediction of the Hypothetical Proteins from Pseudomonas Aeruginosa PA7
Publication year - 2019
Publication title -
archives of infectious diseases and therapy
Language(s) - English
Resource type - Journals
ISSN - 2577-8455
DOI - 10.33140/aidt.03.02.3
Subject(s) - pseudomonas aeruginosa , virulence , biology , computational biology , hypothetical protein , function (biology) , membrane protein , pseudomonas , microbiology and biotechnology , bacteria , integral membrane protein , proteomics , opportunistic pathogen , genome , genetics , gene , membrane
Background & Aim: Pseudomonas aeruginosa is the most frequently isolated bacterium among those gram negative rods thatare obligated aerobes. It is one of the important opportunistic human pathogens, causing severe chronic respiratory infectionin patient with underlying conditions such as cystic fibrosis (CF) or bronchiectasis. The emergence of multi-drug resistancePseudomonas aeruginosa strain in clinically isolated demands the development of better or new drugs against this pathogen.The study is to assign a precise function to hypothetical protein (HPs), whose functions are unknown.Materials and Methods: With the help of various bioinformatics tools, the extensive functional analysis of these hypotheticalproteins was performed. This study combines a number of bioinformatics tools including Blastp, Pfam, InterProScan, SMART,PSLPred, CELLO, Signal Peptide, Expasy’s ProtParam tool, VirulenPred, VicmPred to gain information about the conservedregions, families, pathways, interactions, localizations and virulence related to particular protein.Result: The hypothetical proteins present in Pseudomonas Aeruginosa PA7 genome was extensively analyzed and annotated, outof 1350 hypothetical proteins, 25 proteins are catalytic domains, 31 proteins are enzymes, 46 proteins are integral membraneproteins, 72 proteins are transporters, 104 proteins are binding proteins, 404 proteins sequences contain a domain of unknownfunction (DUF), and 540 proteins cannot be functionally determined by any of the tools.Conclusion: Better understanding of the mechanism of pathogenesis and in finding novel therapeutic targets for Pseudomonasaeruginosa.