Assessments of Antibacterial Photo-Dynamic Inhibition Technique (Apdt) In Combating Multiple Drug Resistant Clinical Isolates (MDRCI)

The purpose of this research is to assess the effect of Antibacterial photo-dynamic inhibition technique(APDT) in combatingmultiple resistance clinical isolates (MDRCI). Theera of antibiotic resistance opened new doors for scientists in the discoveryof new antibiotics and new more chemotherapeutic agents, these discoveries leads to easy treatment of variety of bacterialinfection. For this study, multidrug resistance isolates of Vibrio cholera and Staphylococcus aureus and susceptible strain ofStaphylococcus epidermidiswere used for this research work. All isolates were from clinical sources. Riboflavin (vitamin B2)was used as photo-sensitizer at different logarithmic concentrations ranging from 100 to 104, while the light source was froma UV foto-dyne documentation effecting photons at 300nm. There were controls for all the pathogens used and grouped asblank, light and riboflavin exposed organism were the main focus for this study. It was observed that those treated with UV lightand riboflavin at various concentrations below 100 showed successful inhibition effects on all the clinical pathogenic isolatesassayed at various light exposure. It was also observed that the control group showed different low responses compared withthose treated with UV light, the clinical isolates treated with UV light and riboflavin has high response and considered to berelatively reactive asphoto-dynamic inhibitors. In conclusion, antimicrobial photo-dynamic inhibition therapy may be a usefultools in the inhibition of clinical isolates as modern tools for the eradication of resistance microorganism.