Open AccessHepatoprotective Activity of Amomum Subulatum (Roxb.) Seeds against ParacetamolInduced Liver Damage in Rats
Publication year - 2019
Publication title -
advances in bioengineering and biomedical science research
Language(s) - English
Resource type - Journals
ISSN - 2640-4133
DOI - 10.33140/abbsr.02.02.5
Subject(s) - malondialdehyde , lactate dehydrogenase , catalase , alkaline phosphatase , glutathione , oxidative stress , chemistry , pharmacology , liver damage , superoxide dismutase , biochemistry , enzyme , biology , medicine
Hepatoprotective activity of methanolic extract of Amomum subulatum seeds (MEAS) was evaluated against paracetamol (3 g/kg,p.o. for 3 days) induced liver damage in rats. The degree of protection was measured using serum biomarkers like Alanine andAspartate aminotransferase, alkaline phosphatase, total and direct bilirubin, lactate dehydrogenase, gamma glutamyl transferaseand total protein; oxidative stress parameters such as malondialdehyde, glutathione, super oxide dismutase and catalase alongwith histopathology. Significant higher levels of above serum biomarkers, whereas lower level of total protein in paracetamolcontrol as compared to normal control was observed. Treatments with MEAS and silymarin caused significant reversal in abovebiomarkers which was altered by paracetamol. Similarly, significant increase in MDA while decrease in GSH level, SOD andCAT activities in paracetamol control as compared to normal control. Treatment with MEAS and silymarin brings these stressparameters changes significantly towards normal level as compared to paracetamol control. Histopathological changes werein the same direction supports finding of above biomarkers and stress parameters. These results suggest that MEAS may havepotential therapeutic value to reduce the severity of paracetamol induced liver damage, probably by its antioxidative effect onliver by eliminating the deleterious effects of toxic metabolites of paracetamol.