Open AccessCharacterization of Yeast Strains with Ketoreductase Activity for Bioreduction of Ketones
Author(s) -
Pál Csuka,
László NagyGyőr,
Zsófia Molnár,
Csaba Paizs,
Viktória Bódai,
László Poppe
Publication year - 2021
Publication title -
periodica polytechnica. chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.322
H-Index - 19
eISSN - 1587-3765
pISSN - 0324-5853
DOI - 10.3311/ppch.17429
Subject(s) - yeast , candida parapsilosis , enantiopure drug , ketone , chemistry , pichia , stereoselectivity , candida albicans , microbiology and biotechnology , organic chemistry , biology , biochemistry , catalysis , enantioselective synthesis , recombinant dna , pichia pastoris , gene
This study describes six yeast strains for stereoselective ketone reductions. The reaction conditions for the yeast strains (Pichia carsonii, Lodderomyces elongisporus, Candida norvegica, Candida guillermondi, Debaromyces fabryi and Candida parapsilosis) were optimized in a design of experiments for three ketones of different properties. The pH tolerance, temperature stability and productivity of the bioreductions with lyophilized cells of the yeast strains were characterized. In several cases, the optimized bioreductions resulted in enantiopure alcohols (ee > 99 %) with conversions ranging from moderate to excellent.