Open AccessIsolasi Dan Identifikasi Senyawa Kimia Monoterpen Dari Fraksi Etilasetat Daun Keji Beling (Strobilanthes crispa (L.) Blume) Yang Mempunyai Daya Sitotoksik
Author(s) -
Lilik Sulastri,
Ristanti Mega Lestari,
Partomuan Simanjuntak
Publication year - 2021
Publication title -
jurnal fitofarmaka indonesia/jurnal fitofarmaka indonesia
Language(s) - English
Resource type - Journals
eISSN - 2541-2329
pISSN - 2356-0398
DOI - 10.33096/jffi.v8i1.721
Subject(s) - maceration (sewage) , chemistry , chromatography , ethyl acetate , brine shrimp , fractionation , traditional medicine , column chromatography , hexane , thin layer chromatography , medicine , materials science , composite material
Isolation and identification one of chemical compound mono-terpene from Keji beling plant (local name) (Strobilanthes crispa) has been done. Extraction was carried out by maceration of the keji beling leaf powder with ethanol 96%, then partitioned with water-ethyl acetate. Ethylacetate extract was isolated and purified by a cytotoxic guide fractionation system with Brine Shrimp Lethality Test (BSLT) on column chromatography (SiO2; n-hexane - ethylacetate = 20 : 1); and preparative Thin Layer Chromatography (TLC) (SiO2; n-hexane - ethylaseate = 10 : 1) which gave one compound in oily form. Based on identification by infra red spectra and gas chromatography-mass spectra (GC-MS), the compound is a monoterpene D-limone which has a cytotoxic power of 73,11 ppm.