Open AccessSKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK BUAH BUNI (Antidesma bunius (L.) SPRENG)
Author(s) -
Arif Rahman,
Abd. Malik,
Aktsar Roskiana Ahmad
Publication year - 2016
Publication title -
jurnal fitofarmaka indonesia/jurnal fitofarmaka indonesia
Language(s) - English
Resource type - Journals
eISSN - 2541-2329
pISSN - 2356-0398
DOI - 10.33096/jffi.v3i2.497
Subject(s) - chemistry , antioxidant , radical , quercetin , absorbance , traditional medicine , nitric oxide , ic50 , dpph , food science , flavonoid , organic chemistry , biochemistry , chromatography , in vitro , medicine
Free radicals play a role in the occurrence of various degenerative diseases that require free-radical scavengers or antioxidants. Buni fruit (Antidesma bunius (L.) Spreng) has the bioactive components are Anthocyanins (flavonoids) that serves to the free radicals. This study aimed to measure the antioxidant activity of theethanol extract contained 70% fruit Buni obtained by using the method of nitric oxide. Simplicia buni macerated dried fruit with 70% ethanol. Extracts were obtained in the test antioxidant activity against nitric oxide radicals. The antioxidant activity against free radical absorbance measured by means of UV-Vis spectrophotometry at a wavelength of 546 nm and calcul ated IC50 values. The results shows that the fruit buni has potential as a free radical with IC50 value of 2.28 µg/mL and a comparison of quercetin with IC50 value of 5.88 µg/mL.