Open AccessModifikasi Larutan Buah Bit (Beta vulgaris l.) sebagai Alternatif Pengganti Zat Warna Eosin 2% pada Pemeriksaan Telur Cacing STH (Soil Transmitted Helminths)
Author(s) -
Benediktus Adventhia Daeli,
Fepy Yulianti,
Karolina Rosmiati
Publication year - 2021
Publication title -
borneo journal of medical laboratory technology
Language(s) - English
Resource type - Journals
ISSN - 2622-6111
DOI - 10.33084/bjmlt.v3i2.2397
Subject(s) - eosin , eosin y , pigment , chemistry , population , stain , zoology , helminths , horticulture , biology , food science , staining , botany , biochemistry , medicine , genetics , environmental health , organic chemistry , photocatalysis , catalysis
Helminthiasis is one of the infections caused by parasites. World Health Organization (WHO) data in 2018 reported 24% of the world's population was infected by Soil-Transmitted Helminths (STH). Worms of this group need soil to be in their infective stage. Eosin 2% is a stain used in the examination of worm eggs. Beetroot (Beta vulgaris L.) is one of the plants widely used as a natural dye. Beetroot contains betacyanin which produces red pigment. This study aimed to identify the feasibility of beetroot as a substitute for eosin to stain worm eggs. This research was true experimental with a static group comparison design. The results showed that the concentration of beetroot solution proportional to the quality of 2% eosin is with a concentration of 95%. Beetroot solution produces color quality with a contrasting background so that the morphology of eggs can be clearly observed. Beetroot extract lasts for 7-day storage at cold temperature.