Open AccessApplication of a Real-Time PCR Method for Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens Detection in Water Samples
Author(s) -
Beata Rozwadowska,
Marta Albertyńska,
Grzegorz Hudzik,
Hubert Okła,
Krzysztof Jasik,
Jan Słodki
Publication year - 2013
Publication title -
polish journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.312
H-Index - 34
eISSN - 2544-4646
pISSN - 1733-1331
DOI - 10.33073/pjm-2013-060
Subject(s) - clostridium perfringens , salmonella , microbiology and biotechnology , staphylococcus aureus , escherichia coli , bacteria , biology , clostridium , indicator bacteria , pathogenic bacteria , fecal coliform , water quality , ecology , biochemistry , genetics , gene
The diagnostic assessment of water sanitary state is based mainly on the cultivation of bacteria retained on membrane filters. However classical microbiology methods have a lot of disadvantages. More and more frequently, rapid detection and identification of pathogens present in water is based on molecular biology techniques. The aim of this study was to determine the effectiveness and usefulness of a real-time PCR method, when compared to the recommended bacteria culture method, in diagnostics of pathogens in water samples. The research concerned the detection and identification of main sanitary indicators of water such as: Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens. The analyses were conducted in water samples contaminated with the reference material (the aforementioned bacteria) and real environmental samples, which were examined for the presence of nucleic acid of: Salmonella spp., E. coli, S. aureus and C. perfringens using a real-time PCR method.