Open AccessPrevalence of cagA, vacA, babA2 and iceA Genes in Helicobacter pylori Strains Isolated from Colombian Patients with Functional Dyspepsia
Author(s) -
Azucena Arévalo-Galvis,
Alba Alicia Trespalacios-Rangel,
William Otero,
Marcela Mercado-Reyes,
Raúl A. Poutou-Piñales
Publication year - 2012
Publication title -
polish journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.312
H-Index - 34
eISSN - 2544-4646
pISSN - 1733-1331
DOI - 10.33073/pjm-2012-004
Subject(s) - caga , helicobacter pylori , virulence , genotype , microbiology and biotechnology , gene , medicine , biology , gastroenterology , genetics
The clinical outcome of Helicobacter pylori infection has been particularly associated with virulence genotypes. These genotypes are useful as molecular markers in the identification of patients that are infected and at high risk for developing more severe gastric pathologies. Our main objective was to determine the prevalence of virulence genotypes cagA, vacA, iceA and babA2 of H. pylori, in patients with functional dyspepsia who are infected with the bacteria. H. pylori genotypes babA2 and cagA as well as vacA and iceA allelic variants were identified by PCR in 122 isolates resulting from 79 patients with functional dyspepsia. A high prevalence of genes cagA+ (71%), vacAs1am1 (34%), babA2 (57%) and iceA1 (87%) was found. The most frequent combined genotype found were cagA+/vacAs1am1/babA2+/iceA1 and cagA-/vacAs1am1/babA2+/iceA1, regardless of any family history of gastric cancer or MALT lymphoma. The very virulent genotype cagA+/vacAs1am1/babA2+/iceA1 prevailed in the studied patients with functional dyspepsia. Our results provide information about the prevalence of four of the more important virulent factors and constitute new evidence on the prevalence of the most virulent H. pylori genotype in patients with functional dyspepsia.