Open Accessβ-glucanase Productivity Improvement via Cell Immobilization of Recombinant Escherichia coli Cells in Different Matrices
Author(s) -
Usama Beshay,
Hesham A. ElEnshasy,
Ihab Ismail,
Hassan Moawad,
Sawsan Abd-El-Ghany
Publication year - 2011
Publication title -
polish journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.312
H-Index - 34
eISSN - 2544-4646
pISSN - 1733-1331
DOI - 10.33073/pjm-2011-018
Subject(s) - glucanase , escherichia coli , chemistry , fermentation , matrix (chemical analysis) , bacteria , enzyme assay , enzyme , strain (injury) , recombinant dna , substrate (aquarium) , microbiology and biotechnology , chromatography , biochemistry , biology , ecology , genetics , anatomy , gene
The studies have been performed to analyze the production of beta-glucanase by a recombinant strain of Escherichia coli immobilized in different matrices. Porous sintered glass SIRAN, Ceramic supporting matrices and Broken Pumice stone as well as SIRAN Raschig-rings were examined for the immobilization of whole bacterial cells. The beta-glucanase activity of bacteria immobilized in CeramTec PST 5 (4-5 mm) was very low. CeramTec PST 5 (1.5-2.5 mm) was found to be the best carrier compared to all other matrices regarding glucanase production (630 U/ml) and compared to enzyme activity produced by free cells (500 U/ml). Different doses of matrices were applied (2, 5, 7, 10 g/lask) in the form of "matrix weight". Using 2 g/flask of CeramTec PST 5 (1.5-2.5 mm) yielded enzyme activity of 630 U/ml). CeramTec gives highest operational stability of beta-glucanase by repeated batch fermentation to 5 cycles, and activity reached 660 U/ml. Scanning electron microscopy observations showed a high number of vegetative cells that continued growth inside the matrices, indicating that beta-glucanase activity improvement was due to the immobilization of the cells.