
IN VITRO PROPAGATION OF PLUM ROOTSTOCK DOCERA 6
Author(s) -
Viktorija Nikolova,
AUTHOR_ID,
Vanja I. Akova,
M. Nesheva,
S. Malchev,
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AUTHOR_ID,
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Publication year - 2021
Publication title -
fruit growing research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.1
H-Index - 3
eISSN - 2344-3723
pISSN - 2286-0304
DOI - 10.33045/fgr.v37.2021.18
Subject(s) - rootstock , acclimatization , micropropagation , explant culture , horticulture , biology , auxin , botany , tissue culture , in vitro , biochemistry , gene
This paper describes research on the application of tissue culture techniques to the micropropagation of interspecific rootstock ʹDocera 6ʹ. The experimental work was carried out in the period 2017-2018, in the in vitro propagation laboratory of the Fruit Growing Institute Plovdiv. Аxillary buds were employed as initial explantеs in two different seasons (March-May; September-October). The action of the mineral medium was studied in the multiplication stage. The best result was obtained on LS medium included BAP 0.5 mg/l and IAA 0.05 mg/l. Тhe obtained average multiplication rate is 3.08. The concentration of auxin applied to the basal medium influence the quality of the root system Treatment with high concentrations of IBA added to the rooting medium gives the best results (V5). Тhe influence of the season on growth and development of micropropagated ʹDocera 6ʹ rootstock during ex vitro acclimatization is also part of our research. The spring acclimatization gives better results than the autumn.