Open AccessAN EXPERIMENTAL TRIAL OF BIOPROPHYLACTIC FORMULA DESIGNED TO MINIMIZE COMBINED TOXICITY OF BOTH LEAD AND CADMIUM
Author(s) -
Larisa I. Privalova,
Svetlana V. Klinova,
И. А. Минигалиева,
Iu. V. Ryabova,
М. П. Сутункова,
Oleg H. Makeyev,
Irene E. Valamina,
Tatyana Bushueva,
Svetla. Solovyeva,
В Б Гурвич,
Б А Кацнельсон
Publication year - 2020
Publication title -
gigiena i sanitariâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.275
H-Index - 13
eISSN - 2412-0650
pISSN - 0016-9900
DOI - 10.33029/0016-9900-2020-99-1-85-89
Subject(s) - genotoxicity , cadmium , toxicity , cadmium chloride , nephrotoxicity , in vivo , lead acetate , pharmacology , toxicology , chemistry , medicine , biology , microbiology and biotechnology , organic chemistry
. The increase in the body resistance to the combined effects of lead and cadmium (including systemic toxicity, cardiovascular effects, and genotoxicity) by using a specific bioprotective formula (based on theoretical knowledge and experimental research) remains a pressing challenge. However, a data search has not yielded any results on either an experimental trial or a theoretical justification of the means of biological protection against a variety of adverse effects caused by Pb and Cd combination. Material and methods. The experiment was conducted on the outbred male rats. The animals received repeated intraperitoneal injections of water solutions of lead acetate and cadmium chloride, 3 times per week for 6 weeks. After the exposition was completed, more than 50 indices of toxic exposure (including biochemical and histo-morphological ones) were estimated in all groups of the tested animals. To assess the genotoxic effect of “in vivo” there was used amplified fragment length polymorphism (AFLP) analysis. Statistical analysis was done using Student’s t-test. Results. We found the administration of the bioprotective formula to improve the indices of general toxicity. Genotoxicity studied using AFLP analysis of blood cells DNA was shown to be mitigated. Histo-morphological indices of Pb+Cd hepato- and nephrotoxicity improved under a bioprotective complex (BPC) administration. Blood Pb and Cd decreased during BPC administration. There was a statistically reliable decrease in the mean diameter of cardiomyocytes associated with Pb+Cd administration. These changes became less apparent with the BPC administration. Conclusion. We developed and tested a strategy to mitigate the toxic effects of Pb and Cd at organ and organ system levels, including general toxicity, target organ toxicity (with cardiotoxicity) and genоtoxicity.