Open AccessEvaluation of the Effect of Changing Concentration and pH on The Activity of Rose Bengal Antigen
Author(s) -
Muthanna I. Al-Ezzi
Publication year - 2009
Publication title -
almustansiriya journal of pharmaceutical sciences/al-mustansiriyah journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2959-183X
pISSN - 1815-0993
DOI - 10.32947/ajps.v6i1.360
Subject(s) - rose bengal , diluent , chemistry , chromatography , antigen , bengal , rose (mathematics) , traditional medicine , biology , nuclear chemistry , medicine , immunology , horticulture , geography , organic chemistry , archaeology , bay
The present study was conducted to prepare Rose Bengal antigen by using Rose Bengal stain 1% and brilliant green 0.5%. These antigens were prepared usingdifferent concentrations (4%, 5%, 6%, 7%, 8%, 9% and 10%) and different buffer diluents with different pH (3.0, 3.5, 3.65, 4, 4.5, 5.0) to study the effect of differentantigen concentrations and pH on the Rose Bengal test. It was observed that the best results were obtained when the concentration of the antigen was more than 7% and the pH range of 3.65 to 4.0.