Open AccessDiagnosis of Enteroviral-associated-Meningitis by RT-PCR for the First Time in Iraq
Author(s) -
Faiza L. Tuama,
Yassen I. Omeran,
Manal Khalid Abdul Ridha,
Walid K. Ridha
Publication year - 2008
Publication title -
almustansiriya journal of pharmaceutical sciences/al-mustansiriyah journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2959-183X
pISSN - 1815-0993
DOI - 10.32947/ajps.v5i1.388
Subject(s) - rna extraction , cerebrospinal fluid , rna , meningitis , real time polymerase chain reaction , medicine , extraction (chemistry) , virology , biology , pathology , chromatography , chemistry , gene , surgery , biochemistry
A protocol for testing cerebrospinal fluid specimens using RT-PCR for the diagnosis of central nervous system infections was developed 2 and used to test 20 CSF specimens.Objectives: A-Evaluation of efficiency of In-house prepared Extraction reagent. BAmplified of Enteroviral-RNA genome by single-step RT-PCR. Methods: 20cerebrospinal fluid specimens were included. RNA was extracted from 250μl by method Chomcynki, 1993. EV-RT-PCR was performed for all specimens studied.Results: We successfully isolated total RNA from 20 CSF specimens of AM cases. Ten of 20 specimens were positive by RT-PCR.
Conclusions: Single-step extraction method was allowed isolation of RNA in 4hr. and provided both high yield and purity of un-degraded RNA preparation. EV-RTPCR detection test is suitable for clinical diagnosis of EV-related meningitis and may improve the management of EV-related neurologic syndromes.