Open AccessIn vitro Evaluation of the Effect of Using Different Gelling agents on the Release of Erythromycin from a Nanocubosomal gel
Author(s) -
Sadeq Jaafar Al-Sakini,
Nidhal K. Maraie
Publication year - 2019
Publication title -
almustansiriya journal of pharmaceutical sciences/al-mustansiriyah journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2959-183X
pISSN - 1815-0993
DOI - 10.32947/ajps.v19i1.542
Subject(s) - erythromycin , chemistry , chromatography , spreadability , staphylococcus aureus , poloxamer , in vitro , food science , bacteria , antibiotics , copolymer , organic chemistry , biochemistry , biology , polymer , genetics
Preparation of a topical gel for erythromycin using optimized nano-sized cubosome dispersion, which is changed into a gel to get nanocubosomal gel using different gelling agents. The optimum nanocubosomal dispersion
containing 4.4% glyceryl mono oleate (GMO) (as an oil phase) and 0.6% poloxamer 407 (as an emulsifying agent) was converted into a gel using different gelling agents including carbopol 974, carbopol 940, carbopol 934 and hydroxyl propyl methylcellulose (HPMC) at different ratios of (1:1 and 1:2). Each prepared gel was then evaluated in vitro to determine the homogenisity, consistency, spreadability, viscosity and drug release. The selected formula (FG6) showed best homogenisity, consistency and spreadability as well as it produced initial burst release of 60.3% (within 1 hour) followed by prolonged release of 96.3% continued for 4 hours. In addition, the optimum gel formula (FG6) has shown remarkable antibacterial activity, which was significantly higher than the marketed gel (ERYTHROMYCIN GEL ® 2%) against four different strains of bacteria (Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa).