Study the Expression of Neogene PGBD3 that Derived from DNA Transposons in Colorectal Cancer Cell Lines

The process of molecular domestication that occurred on DNA transposons and gives what is called neogenes that may play an important role in the human genetic instability. One of these Neogene is piggyBac 3 (PGBD3) which is associated with human Cockayne syndrome and Premature ovarian failure. The aim of this work is to study the expression of Neogene PGBD3 in colorectal cancer cell lines. The protein expression of PGBD3 gene have been studied by western blot method in twelve colorectal cancer cell lines (HCT116, SW48, LOVO, DLD1) which are microsatellite instable MSI, (SW480, SW620, HT29, LS123, COLO205, T84, SW403, SW1463) which are microsatellite stable MSS and in healthy tissue of colon as a control. The result of this study showed that the protein expression of PGBD3 gene in all 12 colorectal cancer cell lines were obtained with variable degree of expression which was not seen in healthy colon tissue. From the results of this work, it can be concluded that PGBD3 may have a role in colorectal cancer either in initiation, promotion or progression which need further research and confirmation.