Open AccessIsolation of Mycobacterium tuberculosis and testing their susceptibility to antimicrobial agents by using Bactec 960.
Author(s) -
Afraa Ali Kadhim,
Mohammed Faraj AL-Marjan,
Kadhim Ali Kadhim,
Ahmed Asmar Manki
Publication year - 2013
Publication title -
almustansiriya journal of pharmaceutical sciences/al-mustansiriyah journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2959-183X
pISSN - 1815-0993
DOI - 10.32947/ajps.v13i1.213
Subject(s) - ethambutol , mycobacterium tuberculosis , streptomycin , tuberculosis , microbiology and biotechnology , isoniazid , antimicrobial , drug resistance , medicine , isolation (microbiology) , mycobacterium , antibiotics , biology , pathology
This study was done from 3/7/2011 to 1/1/2012 , different clinical samples were collected from (1235) patients that coming to the Specialized center for chest and respiratory diseases / Baghdad to investigate Mycobacterium tuberculosis and study their resistant to first and second line antimicrobial agents by using Bactec 960.The results obtained from direct smear examination with Ziehl – Neelsen stain in (294) patients that indicated these patients are Infected with tuberculosis . These samples has been cultured on solid medium Lowenstein Jensen media (L.J.) and liquid medium ( middle brook 7H8 broth base) in the Mycobacteria Growth Indicator Tubes (MGIT) using a device (BACTEC MGIT 960 System).Results of (275) samples that represented (22.27%) from total samples were showed a significant real growth that belong to bacteria Mycobacterium tuberculosis. From (275) isolates tested to 1st line anti TB (streptomycin (SM), isoniazid (INH),rifampin (RMP) and ethambutol (EMB)), (196) isolates were resistant to the first line antimicrobial agents that used in the treatment of tuberculosis by using Bactec 960. (125) isolates (63.77%) showed resistance to multiple antimicrobial drugs (MDR-TB).Furthermore, the sensitivity test was done on thirty isolates that characterized by multiple drug resistance criteria (MDR-TB) on second line antimicrobial agents to investigate Extensively Drug-Resistant Tuberculosis(XDR-TB) between these isolates. The results showed that the bestantibiotics in their impact on the isolates (MDR-TB) is (PAS) P-aminosalicylic acid as the percentage of resistance was (3.3%).