Open AccessImproved selection medium and technique for isolation of Azospirillum irakense
Author(s) -
K. M. Khammas,
Nada Z. Mehdi,
A Alwan
Publication year - 2012
Publication title -
almustansiriya journal of pharmaceutical sciences/al-mustansiriyah journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 2959-183X
pISSN - 1815-0993
DOI - 10.32947/ajps.v11i1.235
Subject(s) - microbial inoculant , rhizosphere , isolation (microbiology) , selection (genetic algorithm) , lactulose , botany , biology , chemistry , food science , horticulture , microbiology and biotechnology , bacteria , inoculation , biochemistry , computer science , genetics , artificial intelligence
An improved selection medium and technique for Isolation of Azospirillum irakense was developed . This technique is based on the utilization of free-nitrogensemi-solid medium supplement with disaccharide, lactulose, as a sole source of carbon and energy instead of malate with a pH value of 7.4 .This selected medium was found to encourage the growth of A. irakense alone and to prevent the other species of Azospirillum.The improved medium was superior than the other available media .The technique was simply applied to detect A.irakense, showed a high increase in numbers of A.irakense isolates obtaining from the soil rhizosphere and roots, and decreased the time cost