Improved selection medium and technique for isolation of Azospirillum irakense | Zendy

K. M. Khammas | Zendy; Nada Z. Mehdi | Zendy; Ali Hussein Alwan | Zendy

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Improved selection medium and technique for isolation of Azospirillum irakense

Author(s) -

K. M. Khammas,

Nada Z. Mehdi,

Ali Hussein Alwan

Publication year - 2012

Publication title -

al mustansiriyah journal of pharmaceutical sciences

Language(s) - English

Resource type - Journals

eISSN - 2959-183X

pISSN - 1815-0993

DOI - 10.32947/ajps.v11i1.235

Subject(s) - microbial inoculant , rhizosphere , isolation (microbiology) , selection (genetic algorithm) , lactulose , botany , biology , chemistry , food science , horticulture , microbiology and biotechnology , bacteria , inoculation , biochemistry , computer science , genetics , artificial intelligence

An improved selection medium and technique for Isolation of Azospirillum irakense was developed . This technique is based on the utilization of free-nitrogensemi-solid medium supplement with disaccharide, lactulose, as a sole source of carbon and energy instead of malate with a pH value of 7.4 .This selected medium was found to encourage the growth of A. irakense alone and to prevent the other species of Azospirillum.The improved medium was superior than the other available media .The technique was simply applied to detect A.irakense, showed a high increase in numbers of A.irakense isolates obtaining from the soil rhizosphere and roots, and decreased the time cost

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