Open AccessMetformin Inhibits Growth of Breast Cancer Cell T47 through Decreasing Expression of Protein P53, BCL2 and Cyclin D1
Author(s) -
Irma Yanti Rangkuti,
Poppy Anjelisa Zaitun Hasibuan,
Tri Widyawati,
Yahwardiah Siregar
Publication year - 2019
Publication title -
journal of medical research and innovation
Language(s) - English
Resource type - Journals
ISSN - 2456-8139
DOI - 10.32892/jmri.164
Subject(s) - metformin , cyclin d1 , cell cycle , breast cancer , apoptosis , cancer research , cancer , medicine , endocrinology , oncology , biology , diabetes mellitus , biochemistry
Breast cancer is a disease that afflicts women only 0.5 to 1 % are male breast cancers.Breast cancer has several variants and requires a different therapeutic approach, and until now the therapy has not been satisfactory due to the emergence of resistance. Metformin as the main choice drug type 2 diabetes mellitus which is known to have a cytotoxic effect for breast cancer. This study aimed to analyze metformin cytotoxic mechanisms covering the cell cycle , apoptosis, expression of p53, bcl-2 and cyclin D1 T47D cells which exposed to metformin HCl. The study was conducted invitro on T47D breast cancer cells which exposed to metformin concentrations of 1738.2 µg / mL and 3476.4 µg / mL and doxorubicin concentrations of 0.1µg / mL and 0.2µg / mL for 24 hours. Cell cycle testing and apoptosis using the flowsitometry method and expression test of p53 protein, bcl-2 dancycline D1 in T47D cells with immunocytochemistry. Data was analyzed by one way Anova with Bonferroni's advanced test. The results showed that metformin inhibited the G0-G1 phase of the T47D cell cycle, triggered T47D cell apoptosis, significantly reduced p53, bcl-2 and cyclin D1 protein expression (p <0.05). Conclusion of the study, metformin inhibits T47D cells through inhibition of the cell cycle G0-G1 phase, reducing protein expression p53, bcl-2 and cyclin D1.