Open AccessInhibition of cancer cells using target-specific 2A3 antibody-conjugated gold nanoclusters
Author(s) -
Jui-Chi Kuo,
TsungRong Kuo,
Fajar Rinawati,
Erna Susilowati,
- Sucipto,
Dyah Ika Krisnawati
Publication year - 2022
Publication title -
acta biochimica indonesiana
Language(s) - English
Resource type - Journals
eISSN - 2654-6108
pISSN - 2654-3222
DOI - 10.32889/actabioina.69
Subject(s) - chemistry , cancer cell , viability assay , fluorescence , nanoclusters , biophysics , cell , microbiology and biotechnology , cancer , biochemistry , biology , genetics , physics , organic chemistry , quantum mechanics
Background: Metal nanoclusters (NCs) with outstanding structural and optical properties have been intensively validated for applications in nanomedicine and nanotechnology. Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) is overexpressed in many cancer cells.
Objective: The gold nanoclusters conjugated with a single domain antibody targeting CEACAM6 of 2A3 (2A3-AuNCs) were synthesized for the inhibition of cancer cells.
Methods: 2A3-AuNCs were prepared via a facile hydrothermal approach. The cell viability was measured by resazurin dye reduction assay. The cell death was analyzed by fluorescence imaging.
Results: Structural and optical characterizations demonstrated the successful synthesis of 2A3-AuNCs with a roughly spherical shape and a size of 2.35 nm. The 2A3-AuNCs revealed a maximum fluorescence intensity at 350 nm with a fluorescence quantum yield of 4.0%. The cell viability assay indicated that 2A3-AuNCs could inhibit the growths of cancer cells with overexpressed CEACAM6, including breast cancer MDA-MB-231 and MDA-MB-468 cells. The fluorescence imaging results also demonstrated that 2A3-AuNCs could inhibit the growth of cancer cells with MDA-MB-231 and MDA-MB-468 cells.
Conclusion: Combination with the results of cell viability assay and fluorescence imaging, the surface ligand of 2A3 antibody on 2A3-AuNCs exhibited promising inhibition of CEACAM6 overexpressed cancer cells. Our work provides a potential application of AuNCs in cancer therapy.