Open AccessThe effect of fermented tempeh aerobic anaerobic (FETAA) on pancreatic duodenal homeobox 1 (Pdx1) gene expression and HOMA-beta index in diabetic mice
Author(s) -
Hendi Wicaksono,
Prasetyastuti Prasetyastuti,
Pramudji Hastuti,
Ahmad Hamim Sadewa
Publication year - 2021
Publication title -
acta biochimica indonesiana
Language(s) - English
Resource type - Journals
eISSN - 2654-6108
pISSN - 2654-3222
DOI - 10.32889/actabioina.11
Subject(s) - medicine , endocrinology , pdx1 , insulin , diabetes mellitus , oxidative stress , streptozotocin , beta cell , chemistry , insulin resistance , islet
Background: Diabetes is a result of oxidative stress which causes the impaired function of pancreatic beta-cells. Fermented tempeh aerobic anaerobic (FETAA) containing gamma-aminobutyric acid and isoflavones can reduce oxidative stress in diabetes.
Objective: The aim of this study is to evaluate FETAA in improving pancreatic β-cell function in diabetic mice.
Methods: Twenty streptozotocin-induced diabetic mice, divided into four groups (n = 5 each group): DM, DM + FETAA 10 mg/100 g BW, DM + FETAA 20 mg/100 g BW, DM + FETAA 40 mg/100 g BW as well as normal group (n = 5). DM mice were treated with FETAA for 21 days. Fasting glucose was determined using the GOD-PAP method, while insulin level was determined by ELISA. The homeostasis model assessment of β-cell function (HOMA-β) was calculated using the HOMA2 calculator, and the Pdx1 mRNA level was determined by Real Time-PCR.
Results: The DM mice group treated with FETAA had lower glucose levels than the DM mice group. FETAA dosage of 40 mg/100 g BW was able to reduce the highest blood glucose levels (p 0.05).
Conclusion: FETAA could improve HOMA-β, blood glucose levels, but did not affect Pdx1 mRNA expression.