Open AccessHigh Reusability of Lipase Immobilized on Eggshells for Butyl Butyrate Synthesis under Optimum Condition
Author(s) -
Noor Aziah Serri,
Mooi Lik Sze
Publication year - 2019
Publication title -
asm science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.12
H-Index - 6
eISSN - 2682-8901
pISSN - 1823-6782
DOI - 10.32802/asmscj.2019.247
Subject(s) - candida rugosa , lipase , glutaraldehyde , eggshell , chemistry , propionate , substrate (aquarium) , butyrate , chromatography , yield (engineering) , nuclear chemistry , organic chemistry , materials science , enzyme , fermentation , composite material , ecology , oceanography , biology , geology
Eggshells capacity as supporting material for immobilisation of Candida rugosa Type VII lipase (CRL) was tested. Different parameters for the immobilisation of eggshells were studied including the temperature (30-50°C), pH of buffer the solution (pH 5-8), particle size of the eggshells (≤1mm, 1-2mm and 2-4mm), immobilisation time (1-4 hr) and glutaraldehyde concentration (0.30-3.00%, v/v). The best immobilisation conditions obtained were ≤1mm size of the eggshells added with 0.75% of glutaraldehyde at pH buffer 6. The immobilisation time taken was 3 hr at 50°C. Then optimal conditions for the ester synthesis of butyl butyrate were achieved using 2.0g of the immobilised lipase loading that reacted at 30°C using a 0.2 molar ratio of the substrate for 3 hr. Under optimal conditions (immobilization and esterification), 93.3% of conversion yield of butyl butyrate was obtained. Furthermore, the synthesized immobilized CRL lipase demonstrate that it was highly reusable as it retained 50% of the initial activity after 31 cycles.