Open AccessOvarian Tissue Vitrification as a Method for Ovarian Preservation in Women with Cancer: an Analysis of Granulose Cell Apoptosis
Author(s) -
Budi Wiweko,
Huthia Andriyana,
Achmad Aulia
Publication year - 2016
Publication title -
indonesian journal of obstetrics and gynecology
Language(s) - English
Resource type - Journals
eISSN - 2338-7335
pISSN - 2338-6401
DOI - 10.32771/inajog.v4i2.81
Subject(s) - ovary , vitrification , ovarian tissue , medicine , apoptosis , andrology , ovarian cancer , gynecology , obstetrics and gynaecology , urology , oncology , cancer , biology , pregnancy , biochemistry , genetics
Objective: To obtain the effective method of ovarian function preservation
with granulose cell apoptosis assessment. Ovarian tissue
vitrification became a method for ovarian function preservation in
women with cancer. This technique can be done anytime without
delay on cancer therapy both in prepubertal and unmarried patient.
It can also store many primordial follicles. Ovarian tissue vitrification
study is still limited to animal test and there are no data about apoptosis
assessment after ovarian vitrification in human ovary.
Method: This quasi experimental study was held in Department of
Obstetrics and Gynecology Faculty of Medicine University of Indonesia
- Dr. Cipto Mangunkusumo General Hospital and Fatmawati
Hospital Jakarta from March 2012 to May 2015. Ovaries from thirteen
women between 31 and 37 years old who underwent oophorectomy
with gynecological indication were examined.
Result: There was no morphological difference between follicles
from fresh and warmed-vitrified ovaries. The mean protein Bax
expression on the fresh ovaries assessed in the form of H-score
was 1.66 (SD 0.14) compared with 1.68 (SD 0.13) on the warmedvitrified
group (p=0.165). The mean protein Bcl-2 expression on the
fresh ovaries examined in the form of H-score was 1.73 (SD 0.10)
compared with 1.71 (SD 0.10) on the warmed-vitrified group
(p=0.068).
Conclusion: Ovarian tissue vitrification does not affect the Bax and
Bcl-2 expression on human ovary.
[Indones J Obstet Gynecol 2016; 4-2: 88-92]
Keywords: apoptosis, bax, Bcl-2, ovarian tissue vitrification