Open AccessGlutathione Peroxidase1 Gene Polymorphism (GPx1 Pro198Leu) in Association with Blighted Ovum
Author(s) -
Asiyeh Moshtaghi,
Reyhaneh Sariri,
Hamidreza Vaziri,
Horiyeh Shayegan
Publication year - 2016
Publication title -
indonesian journal of obstetrics and gynecology
Language(s) - English
Resource type - Journals
eISSN - 2338-7335
pISSN - 2338-6401
DOI - 10.32771/inajog.v4i1.66
Subject(s) - restriction fragment length polymorphism , genotype , andrology , glutathione peroxidase , biology , gene , microbiology and biotechnology , medicine , genetics , glutathione , enzyme , biochemistry
Objective: To evaluate salivary GPx-1 gene polymorphism in pregnant
women suffering from blighted ovum.
Method: In this case-control study, 34 blighted ovum patients and
34 healthy controls were studied. Genomic DNA was extracted from
the saliva. The genotypes were determined by restriction fragment
length polymorphism (RFLP-PCR) technique. Mad Calc (version
12.1) was used for statistical analysis.
Result: The frequency of CC, CT, and TT genotypes of GPx-1 gene
were 41%, 44% and 14%, respectively in blighted ovum patients
and in healthy volunteers were 44%, 47%, and 8.82-9%, respectively.
After statistical analysis, the study showed no significant association
between this polymorphism and blighted ovum (with p =
0.63).
Conclusion: These results indicated no significant association between
GPx-1 (Pro198Leu) polymorphism and blighted ovum. However,
further research is required to clarify the role of gene polymorphism
in blighted ovum.
[Indones J Obstet Gynecol 2016; 1: 15-18]
Keywords: abortion, blighted ovum, glutathione peroxidase-1, GPx-
1, RFLP-PCR