Open AccessCreating and Characterizing Genetic Variation in Tilapia through the Creation of an Artificial Center of Origin
Author(s) -
G. A. E. Gall,
Gideon Hulata,
Eric M. Hallerman,
Bernard May,
N. Umiel
Publication year - 2000
Language(s) - English
Resource type - Reports
DOI - 10.32747/2000.7574344.bard
Subject(s) - biology , microsatellite , genetics , genetic linkage , quantitative trait locus , amplified fragment length polymorphism , population , linkage (software) , tilapia , oreochromis mossambicus , evolutionary biology , gene , allele , fish <actinopterygii> , genetic diversity , demography , fishery , sociology
Five stocks of tilapia [oreochromis niloticus (on), red O. niloticus (ROn), O. aureus (Oa), O. mossambicus (Om), and Sarotherodon galilaeus (Sg)] were used to produce two-way (F1), three-way (3WC) and four-way crosses (4WC). Three 4WC groups, containing equal representation of all four species, formed the base population for a new synthetic stock, called an "artificial center of origin" (ACO). Four genomic maps were created using microsatellite and AFLP markers, two from a 3WC family [Om female and (Oa x ROn) male] and two from a 4WC family [(Om x Oas) females and (Sg x On) male]. Sixty-two loci segregating from the female parent of the 3WC mapped to 14 linkage groups while 214 loci from the male parent mapped to 24 linkage groups. Similarly, 131 loci segregating from the female parent of the 4WC mapped to 26 linkage groups and 118 loci from the male parent mapped to 25 linkage groups. Preliminary screening of an F2 and a 4WC family identified a number of loci associated with cold tolerance and body weight. These loci were clustered in a few linkage groups, suggesting they may be indicative of quantitative trait loci.