Open AccessDevelopment and Application of a Molecular Genetic Map for Melon (Cucumis melo)
Author(s) -
Rafael PerlTreves,
Molly Kyle,
Esra Galun
Publication year - 1993
Language(s) - English
Resource type - Reports
DOI - 10.32747/1993.7568094.bard
Subject(s) - cucumis , melon , germplasm , biology , powdery mildew , rapd , population , molecular marker , cultivar , restriction fragment length polymorphism , horticulture , genetics , botany , genetic diversity , genotype , gene , demography , sociology
This project has generated a systematic survey of DNA polymorphism in Cucumis melo. An RFLP and RAPD survey of the major cultivar groups and botanical varieties of this species has been conducted, with the purpose of assessing the degree of molecular variation and phylogenetic relationships within the melon germplasm and, at the same time, develop sets of markets suitable for mapping the melon genome. Additional activities regarding variation in the melon germplasm in fruit traits and regeneration ability have been initiated as well. The necessary populations required for the development of a molecular map of the C. melo genome have been prepared. An F2 that segregated for 4 viral resistances, powdery mildew resitance and sex type has been derived from a PI 414723 x Topmark cross, and a RILs population has been prepared from it. We have confirmed the resistances in the population and have analyzed the genetic relationships between these resistances. Progress toward the construction of a molecular map of C. melo and the development of markers linked to those traits is described. We have so far screened the first few tens of markers in the F2 population, and many additional ones were screened in DNA bulks prepared from such population.