Open AccessTo the study of toxicological parameters of the medication “Vitosept” in conditions of acute toxicity
Author(s) -
M. P. Soltys,
В М Гунчак
Publication year - 2018
Publication title -
naukovij vìsnik lʹvìvsʹkogo nacìonalʹnogo unìversitetu veterinarnoï medicini ta bìotehnologìj ìmenì s.z. g̀žicʹkogo
Language(s) - English
Resource type - Journals
eISSN - 2518-1327
pISSN - 2413-5550
DOI - 10.32718/nvlvet8821
Subject(s) - chemistry , respiratory tract , albumin , gastrointestinal tract , sodium , toxicity , pharmacology , acute toxicity , respiratory system , physiology , biochemistry , medicine , organic chemistry
The authors examined the toxicological parameters of the drug “Vitosept”, based on a solution sodium chloride, which is obtained by the diaphragm free (diaphragmless) continuous flow electrolyzer. According to the developers, the sodium chloride solution obtained in this way is the optimal carrier of active oxygen; non-toxic; it easily releases active oxygen and penetrates through protein barriers. In the course of the research, it was found that during the whole observation period, the experimental animals were active, had satisfactory appetite, responded to sound and light stimuli, and retained reflex excitability. Clinical signs of violations on the part of the respiratory and urinary tract systems, as well as disorders of the gastrointestinal tract – were absent. Inadequate reactions and death of animals – were not observed. The morphological and biochemical changes in the blood of experimental rats showed an increase in the total number of leukocytes by 19.4%, 28.6% and 33.7%, as compared to control. The erythrocyte index of intoxication in rats of the IVth experimental group increased more than twice (P < 0.05). The total protein content of blood serum of animals of the II and III groups was higher than the control values by 9.3% and 3.2%. At the same time, the increase in albumin fractions was 33.2% and 36.4%, respectively (P < 0.05). Consequently, in the course of the research, it was found that the DL50 of the drug for intragastric (rat) and intra-abdominal (white mouse) inputting is less than 1000 mg/l, and according to this index it relates it to grade IV toxicity – low-toxic compounds. The revealed individual changes in the evaluation of hematological and biochemical parameters of blood in rats and mice did not go beyond the limits of physiological parameters and most likely were compensatory in nature.