Open AccessIn vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract
Author(s) -
Cristiane Pimentel Victório,
Davi Oliveira e Silva,
Daniela Sales Alviano,
Celuta Sales Alviano,
Ricardo Machado Küster,
Celso Luiz Salgueiro Lage
Publication year - 2021
Language(s) - English
Resource type - Journals
ISSN - 2446-4775
DOI - 10.32712/2446-4775.2021.1037
Subject(s) - chemistry , ethyl acetate , antimicrobial , dichloromethane , alpinia , agar diffusion test , chromatography , traditional medicine , botany , biology , organic chemistry , biochemistry , solvent , medicine , escherichia coli , gene
This study aimed to evaluate the in vitro antimicrobial activity of fractions of leaf hydroalcoholic crude extracts: hexane, dichloromethane, ethyl acetate and butanolic of Alpinia zerumbet and A. purpurata by the agar drop diffusion method in order to screen the main compounds involved in antimicrobial activity. Leaves of A. zerumbet (Pers.) B.L. Burtt et R.M. Sm. and A. purpurata (Vieill) K. Schum adult plants were collected and then dried and macerated in 70% ethanol. Leaf extracts were further partitioned using solvents in increasing polarity. Dichloromethane fractions were analyzed by gas chromatography/mass spectrometry (GC/MS). The major compound in the dichloromethane fraction of both species was the hexadecanoic acid (palmitic acid). By drop diffusion assay, the antimicrobial activity of crude extract, as well as fractions of hexane, dichloromethane, ethyl acetate and butanolic, was evaluated against pathogenic bacteria and fungi. No bacteria were inhibited. However, the dichloromethane fraction exhibited promising antifungal activity against the following fungi tested: Cryptococcus neoformans, Fonsecaea pedrosoi, Trichophytoon rubrum, Microsporium canis and M. gypseum.