君迁子离体茎段的组培生根诱导 | Zendy

馨如 耿 | Zendy

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君迁子离体茎段的组培生根诱导

Author(s) -

馨如耿

Publication year - 2020

Publication title -

国际教育论坛

Language(s) - Uncategorized

Resource type - Journals

eISSN - 2705-120X

pISSN - 2705-1196

DOI - 10.32629/jief.v2i3.824

Subject(s) - computer science

为筛选适合君迁子组培生根的最佳激素条件建立君迁子组培快繁体系。本研究利用三年生君迁子实生苗的离体新梢为外植体采用三因素三水平的研究方法研究了在1/2MS培养基中加入不同浓度的不同激素后对君迁子离体组培生根的作用效果。结果表明不带梢尖的幼嫩新梢是最佳的试验材料生根效果最好的条件是1L培养基中加入0.5mgIAA+1/2MS2.17g+琼脂粉5.6g+蔗糖26.6g+PVP0.5g。此条件下三个月后君迁子离体组织的生根率达到22.22%以上平均根长33.18mm。该结论为君迁子组培生根并实现君迁子矮化改良奠定基础。

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