Open AccessDevelopment and Validation of Reverse-phase High-performance Liquid Chromatography Method for Novel Synthetic Pyridine Derivative
Author(s) -
R. S. Dave,
Vipul J. Vyas,
Bipinchandra Mahyavanshi
Publication year - 2021
Publication title -
international journal of scientific research in science and technology
Language(s) - English
Resource type - Journals
eISSN - 2395-602X
pISSN - 2395-6011
DOI - 10.32628/ijsrst2183127
Subject(s) - pyridine , high performance liquid chromatography , derivative (finance) , nitrobenzene , chemistry , solvent , chromatography , sulfonyl , catalysis , organic chemistry , alkyl , financial economics , economics
The study was focused toward synthesis, characterization and quantification of Pyridine derivative by Reverse Phase High Performance Liquid Chromatography method. The synthesis of novel pyridine derivative was carried out by condensation reaction in presence of inert catalyst K2CO3 and THF solvent. Characterization was done by I.R, 1H-NMR,and HPLC. Based on the spectral data, the structure of novel pyridine derivative was characterized as 5 - (4 - Substituted amino) - 3- Nitrobenzene - 1 - Sulfonyl) - 4, 5, 6, 7 –Tetrahydrothieno[3, 2 - C] Pyridine The method validation including precision, accuracy, LOD, robustness, Linearity, and range was developed as per ICH guideline through an efficient isocratic RP-HPLC.It was determined that the above procedure is specified, accurate, precise, robust, and sturdy.