Open AccessDiversity of Endophtic Bacteria in Aerides crispa, an Epiphytic Orchid
Author(s) -
S Darsha,
M Jayashankar
Publication year - 2021
Publication title -
international journal of scientific research in science and technology
Language(s) - English
Resource type - Journals
eISSN - 2395-602X
pISSN - 2395-6011
DOI - 10.32628/ijsrst21822
Subject(s) - biology , orchidaceae , epiphyte , bacillus pumilus , botany , bacteria , bacillus (shape) , genbank , 16s ribosomal rna , firmicutes , bacillus megaterium , microbiology and biotechnology , genetics , gene
Aerides crispa is an epiphytic flowering orchid belongs to the family Orchidaceae.we have identified five different endophytic bacteria from this orchid. Genotypic characterization of the bacterial culture was done using 16S rDNA sequencing after PCR amplification. These sequences were compared with the known similar bacterial sequences from the NCBI GenBank database. Homological relationship of the bacteria was also compared by Phylogenetic tree generation. Bacteria obtained were Bacillus pumilus MT463728, Bacillus megaterium MT540506, Lysinibacillus fusiformis MT540507, Bacillus cereus MT540510, Aneurinibacillus migulanus. From the study, we have molecularly characterized and documented five different endophytic bacteria from the epiphytic orchid, A crispa. This shows the diversity of endophytes in orchids which speaks a lot about the urgent necessity of bioconservation without words.