Open AccessImmobilization of Glucose Oxidase on Polypropylene Micro Test Plate
Author(s) -
Azmi Naqvi,
Dinesh C Sharma
Publication year - 2020
Publication title -
international journal of scientific research in science and technology
Language(s) - English
Resource type - Journals
eISSN - 2395-602X
pISSN - 2395-6011
DOI - 10.32628/ijsrst207650
Subject(s) - glucose oxidase , polypropylene , plate reader , scanner , materials science , immobilized enzyme , chemistry , chromatography , computer science , enzyme , optics , nanotechnology , composite material , artificial intelligence , biosensor , biochemistry , fluorescence , physics
In this paper, we report a precise, reliable, cost effective and sensitive method of immobilization of Glucose oxidase GOx) onto polypropylene microtest plate (PPµTP). Assaying of the immobilized enzyme is carried out by spectrophotometer ¬free image¬ based method. PPµTP acts as a mini reaction and analysis device, scanner as an image capturing instrument and color saturation as an image analytical tool for image – based assay of immobilized GOx.