Open AccessSpectrophotometric Determination of Apixaban in Bulk Drug and Oral Dosage Formulation
Author(s) -
Deepak Sharma,
Vikramsinh R. Chauhan,
K. B. Vyas
Publication year - 2019
Publication title -
international journal of scientific research in science and technology
Language(s) - English
Resource type - Journals
eISSN - 2395-602X
pISSN - 2395-6011
DOI - 10.32628/ijsrst207263
Subject(s) - chromogenic , reagent , chromatography , relative standard deviation , dosage form , pharmaceutical formulation , apixaban , beer–lambert law , spectrophotometry , drug , materials science , chemistry , analyte , detection limit , medicine , pharmacology , rivaroxaban , organic chemistry , optics , physics , warfarin , cardiology , atrial fibrillation
A simple, rapid, cost effective and extractive UV-Vis spectrophotometric method has been developed for the determination of Apixaban (AP) in bulk drug and pharmaceutical formulation. It was based on UV-Vis spectrophotometric measurements in which the drug reacts chromogenic reagent (N-(1-napthyl) ethylene diamine dihydrochloride solution) in acidic medium and give stable pale yellow colored complex which exhibits absorption maximum at 680 nm. Beer’s law was obeyed in the concentration range of 5 - 50 μg /ml. This method was tested and validated for various parameters according to ICH guidelines. The proposed method was successfully applied for the determination of AP in oral formulation. The results demonstrated that the procedure is accurate, precise and reproducible (relative standard deviation < 2 %). As it is simple, cheap and less time consuming, it can be suitably applied for the estimation of LA in dosage forms in quality control labs.