Open AccessAssay of Clopidogrel by Using HPLTC Method
Author(s) -
D. S. Ghotekar,
Vishal N Kushare
Publication year - 2018
Publication title -
international journal of scientific research in science and technology
Language(s) - English
Resource type - Journals
eISSN - 2395-602X
pISSN - 2395-6011
DOI - 10.32628/ijsrst12848252
Subject(s) - absorbance , silica gel , chemistry , toluene , chromatography , stationary phase , analytical chemistry (journal) , triethylamine , thin layer chromatography , calibration curve , methanol , detection limit , organic chemistry
The present paper describes stability indicating high-performance thin-layer chromatography (HPTLC) assay method for clopidogrel in bulk drugs. The method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of toluene: methanol: triethylamine (6.5: 4.0: 0.1 v/v/v). The system was found to give compact spot for clopidogrel ( R f value of 0.40 ± 0.010). Densitometric analysis of Clopidogrel was carried out in the absorbance mode at 254 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r 2 = 0.888 with respect to peak area in the concentration range 30 - 120 ng/spot.