Open AccessBiodegradation of used Engine Oil Contaminated Site using Individual and Mixed bacterial isolates
Author(s) -
A J Muh'd,
A Y Sa'adatu,
M M Surayya,
M I Sa'adatu,
B Nafisa,
S D Hauwa
Publication year - 2020
Publication title -
international journal of scientific research in science, engineering and technology
Language(s) - English
Resource type - Journals
eISSN - 2395-1990
pISSN - 2394-4099
DOI - 10.32628/ijsrset196658
Subject(s) - bacillus cereus , contamination , bioremediation , biodegradation , environmental remediation , bacillus (shape) , soil contamination , micrococcus , microorganism , bacteria , environmental chemistry , pollution , food science , environmental science , biology , microbiology and biotechnology , chemistry , ecology , genetics
Oil is most widely distributed source of energy in the world and large-scaled environmental pollutant. Oil, oil products, and oil containing industrial waste pollution is ranked second place after radioactive pollution on account of their harmful action to ecosystems. Contamination of soil by organic chemicals (mostly hydrocarbons) is prevalent in oil producing and industrialized countries of the world. Biodegradation, a strategy that uses biological means (i.e microbes) to degrade, stabilize and remove soil contaminants is an alternative green technology remediation of hydrocarbon contaminated soil. This study was carried out to isolate and screen Bacteria capable of degrading used engine oil from oil contaminated site. Soil samples were collected from oil contaminated site. Bacterial species were isolated from the collected soil samples by serial dilution and agar methods. Different bacterial species were isolated but only four were oil degrading isolates. The identity of the various genera of bacterial contaminants were determined by a combination of cultural, morphological as well as preliminary biochemical characteristics of the isolates. The four oil degrading bacteria (AJ1, AJ2, AJ3 and AJ4) were preliminarily identified as Bacillus cereus, Pseudomonas aeruginosa, Bacillus subtillis and Micrococcus spp respectively. The degradation ability of the bacterial isolates was screened and maximum degradation was recorded by AJ 5 (Mixed culture) with 66.9 %, followed by Bacillus cereus (50.3 %), Bacillus subtilis (44 %), Pseudomonas aeruginosa (37.9 %) and the least was seen in Micrococcus spp (35.3 %). These findings revealed that some bacteria species are capable of utilizing the oil and used it as sole source of carbon and energy and the mixed consortia of the bacteria have rapid degradation ability. Biological degradation of hydrocarbon contaminated soil offers a better and more environmentally friendly technique that if properly explored can bring our environment into a better place for both plant and animal.