Open AccessInvestigation of Constituents of Oil from the Seeds of Moringa oleifera Plant and Its Biological Activity
Author(s) -
Bilya Abdu
Publication year - 2019
Publication title -
international journal of scientific research in science, engineering and technology
Language(s) - English
Resource type - Journals
eISSN - 2395-1990
pISSN - 2394-4099
DOI - 10.32628/ijsrset19652
Subject(s) - moringa , aspergillus niger , antimicrobial , maceration (sewage) , chemistry , aspergillus flavus , oleic acid , gas chromatography , potency , gas chromatography–mass spectrometry , essential oil , food science , candida albicans , chromatography , botany , mass spectrometry , biology , organic chemistry , biochemistry , materials science , in vitro , composite material
Oil of the dry seeds of Moringa oleifera was obtained by maceration using n- Hexane. GC/MS analysis was performed using a shimadzu QP2010 plus series gas chromatography coupled with shimadzu mass spectroscopy detector. Characterization of constituents was done by comparison with the MS library (NIST) and further confirmed by interpreting the observed fragmentation pattern. Comparison of the mass spectra with the database on MS library revealed about 90-95% match. The most common major constituents of the oil include: 10-Octadecenoic acid methyl ester (48.78%), Octadecanoic acid methyl ester (7.97%), Methyl ricinolleate (14.59%).The antimicrobial screening of the oil was also performed: Strong antimicrobial potency was shown against Staphylococus aureus. The oil was also active against the fungal species Aspergillus niger. Significant antifungal potency against Candida albicans was also observed. Minimum inhibition concentration and minimum bactericidal/ fungicidal activities were also evaluated for the sample: minimum inhibition concentration was recorded at 12.50mg/ml for all the test organisms except Bacillius cereus and Escherachia coli. Minimum bactericidal potential against test organisms at 50mg/ml was observed for morimga oleifera seed oil.