Open AccessEVALUATION OF ANTIOXIDANT ACTIVITY OF CHLOROPHYLL
Author(s) -
D. Acharya,
Umesh Kumar Gilhotra,
Ranjan Singh,
Himanshu Rajpurohit,
Ajay Gar
Publication year - 2019
Publication title -
journal of biomedical and pharmaceutical research
Language(s) - English
Resource type - Journals
eISSN - 2589-8752
pISSN - 2279-0594
DOI - 10.32553/jbpr.v8i6.676
Subject(s) - antioxidant , dpph , ascorbic acid , chemistry , hydrogen peroxide , chlorophyll , food science , biochemistry , organic chemistry
Antioxidant play an important role to protect damage caused oxidative stress (OS).Chlorophyll having phenolic contents are reported to possess antioxidant properties. The present study was designed to investigate the antioxidant properties of methanolic solution from Super Chlorophyll.
Objective: The main objective of the study was to evaluate the antioxidant activity of the chlorophyll in different validated in vitro models.
Methods: The antioxidant activities of methanolic solution were evaluated by in vitro standard method using UV-Spectrophotometer. The antioxidant activity were determined bt total antioxidant capacity, DPPH (1-1-diphennyl-2-picrylhydrzine) radical scavenging assay, Nitric Oxide scavenging assay and Hydrogen Peroxide scavenging assay methods.
Result: The solution of Chlorophyll was studied for antioxidant potential. Ascorbic acid was used as standard. This method is simple and activity of the solution is reported in term of IC50 value. Antioxidant properties depend on the IC50 value. Lesser the IC50 value, more is the antioxidant activity. Chlorophyll showed good antioxidant activity.
Conclusion: In case of in vitro antioxidant activity, Chlorophyll scavenged DPPH, hydrogen peroxide, nitric oxide radicals significantly, showed IC50 values near to standard ascorbic acid, thus proving to have good antioxidant potential.
Keywords: Oxidative stress, DPPH, Antioxidant, Super chlorophyll.