Open AccessEnzyme Linked Immunosorbent Assay (ELISA) Technique Guideline
Author(s) -
Rachmat Hidayat,
Patricia Wulandari
Publication year - 2021
Publication title -
bioscientia medicina
Language(s) - English
Resource type - Journals
ISSN - 2598-0580
DOI - 10.32539/bsm.v5i5.228
Subject(s) - antibody , antigen , enzyme , chemistry , microbiology and biotechnology , peptide , substrate (aquarium) , biochemistry , biology , immunology , ecology
ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the form of a color change will be formed due to the reaction between the enzyme and the substrate.