Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline | Zendy

Rachmat Hidayat | Zendy; Patricia Wulandari | Zendy

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Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline

Author(s) -

Rachmat Hidayat,

Patricia Wulandari

Publication year - 2021

Publication title -

bioscientia medicina journal of biomedicine and translational research

Language(s) - English

Resource type - Journals

ISSN - 2598-0580

DOI - 10.32539/bsm.v5i5.228

Subject(s) - antibody , antigen , enzyme , chemistry , microbiology and biotechnology , peptide , substrate (aquarium) , biochemistry , biology , immunology , ecology

ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the form of a color change will be formed due to the reaction between the enzyme and the substrate.

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