Open AccessEffects of some precursors and elicitors on the growth and α-glucosidase inhibitory activity of in vitro Urena lobata L. hairy roots
Author(s) -
Phuong Thi Bach Vu,
Dai Minh Cao,
Phuong Ngo Diem Quach
Publication year - 2020
Publication title -
khoa học và công nghệ: tự nhiên
Language(s) - English
Resource type - Journals
ISSN - 2588-106X
DOI - 10.32508/stdjns.v4i3.902
Subject(s) - methyl jasmonate , salicylic acid , in vitro , phenylalanine , biochemistry , chemistry , biology , botany , amino acid , gene
Recent studies have demonstrated the potential of in vitro Urena lobata L. hairy roots to inhibit α-glucosidase for supporting the treatment of type 2 diabetes. To increase the productivities of hairy roots with α-glucosidase inhibitory activity in in vitro culture conditions, this study focus on the effects of some metabolic factors such as precursors (L-phenylalanine, L-tyrosine) and elicitors (chitosan, methyl jasmonate, salicylic acid). They were added to the culture media to investigate the growth and α-glucosidase inhibitory activity of Urena lobata L. hairy roots. The results showed that for the effects of precursors, only phenylalanine (1 µM) increased root biomass with the highest of α-glucosidase inhibitory activity on the 25th day of culture. In contrast, tyrosine did not play any role in increasing the biomass and α-glucosidase inhibitory activity in Urena lobata L. hairy roots. For the effects of elicitors, only chitosan (50 mg/L) resulted in hairy roots with α-glucosidase inhibitory activity higher than the control after 3 days in culture medium. Other elicitors such as methyl jasmonate, salicylic acid had lower α-glucosidase inhibitory activity than the control. The results of this study demonstrated the potential of phenylalanine and chitosan in increasing the productivity of in vitro hairy roots with higher α-glucosidase inhibitory activity in Urena lobata L.